screening of huge libraries with 100% coverage
Our technology converts your target molecule, new or altered catalytic activity or other desired phenotype into an
optical signal and allows DIRECT detection of the most beneficial clone, you are searching for.
Using common technologies limits your screening to a few Thousands of clones, which does not make 1% of your library.
SenseUP screens whole-libraries and
gives you access to 100% of your
We develop for your phenotype a genetically encoded metabolite sensor, which transforms your desired phenotype
(a target molecule, enzyme activity, protein secretion) into a graded optical output. This allows us to use flow-cytometry
for your project, which boosts screening throughput by the factor 1000x and guaranties the highest
chance, to find your desired strain in months.
Let´s make an experiment together. In the following video you see the analysis of 10.000 of cells in every second.
Each dot represents a measured analysed cell. The higher the cells appear, the more fluorescence is emitted.
The best ones are sorted out immediatly and spotted into a 96-well plate as single cell.
During the time you used to drink your coffee we already would have analysed more than 1.000.000 bacterial cells. More than 40 very promising clones have been sorted out immediately and are waiting now for further analysis.
The chance that one of them already boosts your production process is higher, than ever before.
The technology can be used for nearly all kinds of organisms which are cultivatable under laboratory conditions
We develop biosensors on basis of transcriptional regulators, which sense molecules within a cell by nature.Nearly all
phenotypes are characterized through certain transcription patterns, therefore the development of biosensors against nearly all substances can be possible
of your project by one of our scientists
We successfully developed biosensors for the detection of nearly all industrially relevant amino acids arginine, histidine, lysine, methionine, threonine, valine … and use them for the construction and screening of high productive amino acid production strains.
Another type of sensors converts the phenotype “high protein secretion” into fluorescence and has been used successfully for the screening of highly productive enzyme producers.
For screening of new catalytic activities we have available a new sensor for the measurement of NADPH-consumption within E. coli. This sensor preferably is used for the screening of improved catalytic activities of NADPH-consuming enzymes.
Natural evolution of a Corynebacterium glutamicum
strain for amino acid production with the
Aim: Identification of L-lysine producing clones showing
increased productivity under process conditions.
In this project we doubled the productivity of C. glutamicum by eight simple enrichments of 100.000 promising clones in each step. The screening takes about 2 hours followed by two days cultivation steps.
After two weeks 96 single cells were isolated onto agar plates and analysed individually (two weeks). 70% of them showed increased productivity, three of them about 2x fold.
In one example the genome sequence showed the complete duplication of an operon of the pentose-phophate pathway.
Novel mutations for
hyper-production of L-Lysine in
identified. with the
Aim: Identification of novel mutations leading to l-lysine formation.
We performed random mutagenesis of wild type cells and screened about 10.000.000 cells with our lysine sensor to isolate strains carrying mutations, which turn wild-type cells into l-lysine producers.
After isolation of 40 promising clones we sequenced ten of them, showing more than 150 mutations per strain. For the identification of the relevant ones we re-engineered 200 of them using MULTIPLEX-genome engineering combined with FACS screening.